Comment on “Organelles Ca²⁺ redistribution contributes to cadmium-induced EMT of renal cancer cells through p-cPLA2-mediated arachidonic acid release”
This commentary proposes enhancements to improve a study on cadmium-induced EMT in renal cancer cells. Key recommendations encompass the following aspects: Supplementing statistical power justification through explicit definition of parameters including ≥80% desired power, minimal effect size, and i...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-09-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325010875 |
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| Summary: | This commentary proposes enhancements to improve a study on cadmium-induced EMT in renal cancer cells. Key recommendations encompass the following aspects: Supplementing statistical power justification through explicit definition of parameters including ≥80% desired power, minimal effect size, and intra-group variability; Report exact P values with confidence intervals to improve statistical transparency; Clarification of p38 MAPK versus cPLA2 pathway contributions via co-inhibition experiments; Exclusion of TRP channel confounding effects when using 2-APB through targeted TRPV6 inhibition; Distinction between direct Cd²⁺ effects and toxicity-induced compensatory responses; Provision of explicit rationale for selecting Mag-Fluo-4/AM and Rhod-2/AM fluorescent probes; Experimental verification of PMCA inhibition synergism with cadmium-induced cytosolic Ca²⁺ overload. These recommendations would enhance the robustness of the original study's conclusions. |
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| ISSN: | 0147-6513 |