Multiscale Vascular Enhancement Filter Applied to <italic>In Vivo</italic> Morphologic and Functional Photoacoustic Imaging of Rat Ocular Vasculature
Optical-resolution photoacoustic microscopy (OR-PAM) is used for <italic>in vivo</italic> imaging of a variety of albino and pigmented eyes taking advantages of requiring no exogenous dye, performing high-resolution imaging, and achieving morphologic and functional imaging at the same ti...
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IEEE
2019-01-01
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| Series: | IEEE Photonics Journal |
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| Online Access: | https://ieeexplore.ieee.org/document/8879490/ |
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| author | Huangxuan Zhao Ke Li Ningbo Chen Kunya Zhang Lidai Wang Riqiang Lin Xiaojing Gong Liang Song Zhicheng Liu Chengbo Liu |
| author_facet | Huangxuan Zhao Ke Li Ningbo Chen Kunya Zhang Lidai Wang Riqiang Lin Xiaojing Gong Liang Song Zhicheng Liu Chengbo Liu |
| author_sort | Huangxuan Zhao |
| collection | DOAJ |
| description | Optical-resolution photoacoustic microscopy (OR-PAM) is used for <italic>in vivo</italic> imaging of a variety of albino and pigmented eyes taking advantages of requiring no exogenous dye, performing high-resolution imaging, and achieving morphologic and functional imaging at the same time. However, to accurately diagnose the ophthalmic disease in the OR-PAM images, vascular enhancement algorithms are necessary for extracting vessels and quantifying them correctly. Vascular enhancement algorithms developed for other imaging technologies, are not suitable to be used for OR-PAM, because of the underlying differences in the physics of the formation of images. In this study, a new vascular enhancement algorithm called photoacoustic imaging vasculature enhancement filter (PAIVEF) is proposed, which not only enhances vasculature including micro-vessels signals, suppresses noise signals effectively, but also achieves highly sensitive and accurate enhancement of the vasculature within a large depth range in and out of the system's depth of focus (DOF). Using the PAIVEF, the morphologic and functional 3D images of the whole rat's ocular anterior vasculature segment was displayed simultaneously for a depth range of ∼0.6 mm, which was ∼7 times of the system's DOF. This study paves the way for the application of OR-PAM technology in ophthalmic disease research. |
| format | Article |
| id | doaj-art-02ea3a0519e64cb48537f272dfb62be1 |
| institution | Matheson Library |
| issn | 1943-0655 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | IEEE |
| record_format | Article |
| series | IEEE Photonics Journal |
| spelling | doaj-art-02ea3a0519e64cb48537f272dfb62be12025-07-01T23:51:59ZengIEEEIEEE Photonics Journal1943-06552019-01-0111611210.1109/JPHOT.2019.29489558879490Multiscale Vascular Enhancement Filter Applied to <italic>In Vivo</italic> Morphologic and Functional Photoacoustic Imaging of Rat Ocular VasculatureHuangxuan Zhao0https://orcid.org/0000-0002-9995-7528Ke Li1Ningbo Chen2https://orcid.org/0000-0002-0352-3419Kunya Zhang3Lidai Wang4Riqiang Lin5Xiaojing Gong6https://orcid.org/0000-0002-8221-3522Liang Song7Zhicheng Liu8Chengbo Liu9https://orcid.org/0000-0001-7565-2044School of Biomedical Engineering, Capital Medical University, Beijing, ChinaSchool of Biomedical Engineering, Capital Medical University, Beijing, ChinaResearch Laboratory for Biomedical Optics and Molecular Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, ChinaSchool of Biomedical Engineering, Capital Medical University, Beijing, ChinaDepartment of Mechanical and Biomedical Engineering, City University of Hong Kong, 83 Tat Chee Ave, Hong Kong SAR, Kowloon, ChinaResearch Laboratory for Biomedical Optics and Molecular Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, ChinaResearch Laboratory for Biomedical Optics and Molecular Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, ChinaResearch Laboratory for Biomedical Optics and Molecular Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, ChinaSchool of Biomedical Engineering, Capital Medical University, Beijing, ChinaResearch Laboratory for Biomedical Optics and Molecular Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, ChinaOptical-resolution photoacoustic microscopy (OR-PAM) is used for <italic>in vivo</italic> imaging of a variety of albino and pigmented eyes taking advantages of requiring no exogenous dye, performing high-resolution imaging, and achieving morphologic and functional imaging at the same time. However, to accurately diagnose the ophthalmic disease in the OR-PAM images, vascular enhancement algorithms are necessary for extracting vessels and quantifying them correctly. Vascular enhancement algorithms developed for other imaging technologies, are not suitable to be used for OR-PAM, because of the underlying differences in the physics of the formation of images. In this study, a new vascular enhancement algorithm called photoacoustic imaging vasculature enhancement filter (PAIVEF) is proposed, which not only enhances vasculature including micro-vessels signals, suppresses noise signals effectively, but also achieves highly sensitive and accurate enhancement of the vasculature within a large depth range in and out of the system's depth of focus (DOF). Using the PAIVEF, the morphologic and functional 3D images of the whole rat's ocular anterior vasculature segment was displayed simultaneously for a depth range of ∼0.6 mm, which was ∼7 times of the system's DOF. This study paves the way for the application of OR-PAM technology in ophthalmic disease research.https://ieeexplore.ieee.org/document/8879490/Photoacoustic imagingvascular enhancement filtermorphologic and functional imagingocular vasculature. |
| spellingShingle | Huangxuan Zhao Ke Li Ningbo Chen Kunya Zhang Lidai Wang Riqiang Lin Xiaojing Gong Liang Song Zhicheng Liu Chengbo Liu Multiscale Vascular Enhancement Filter Applied to <italic>In Vivo</italic> Morphologic and Functional Photoacoustic Imaging of Rat Ocular Vasculature IEEE Photonics Journal Photoacoustic imaging vascular enhancement filter morphologic and functional imaging ocular vasculature. |
| title | Multiscale Vascular Enhancement Filter Applied to <italic>In Vivo</italic> Morphologic and Functional Photoacoustic Imaging of Rat Ocular Vasculature |
| title_full | Multiscale Vascular Enhancement Filter Applied to <italic>In Vivo</italic> Morphologic and Functional Photoacoustic Imaging of Rat Ocular Vasculature |
| title_fullStr | Multiscale Vascular Enhancement Filter Applied to <italic>In Vivo</italic> Morphologic and Functional Photoacoustic Imaging of Rat Ocular Vasculature |
| title_full_unstemmed | Multiscale Vascular Enhancement Filter Applied to <italic>In Vivo</italic> Morphologic and Functional Photoacoustic Imaging of Rat Ocular Vasculature |
| title_short | Multiscale Vascular Enhancement Filter Applied to <italic>In Vivo</italic> Morphologic and Functional Photoacoustic Imaging of Rat Ocular Vasculature |
| title_sort | multiscale vascular enhancement filter applied to italic in vivo italic morphologic and functional photoacoustic imaging of rat ocular vasculature |
| topic | Photoacoustic imaging vascular enhancement filter morphologic and functional imaging ocular vasculature. |
| url | https://ieeexplore.ieee.org/document/8879490/ |
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