In Vitro Efficacy of Water-Soluble Mercaptopyridine-Substituted Oxotitanium (IV) Phthalocyanine Compounds in Photodynamic Therapy of Oral Squamous Cell Carcinoma
<i>Background and Objectives</i>: The efficacy of newly synthesized water-soluble octa-mercaptopyridine-substituted oxotitanium (IV) phthalocyanine (oxo-TiPc) and copper (II) phthalocyanine (CuPc) compounds in photodynamic therapy (PDT) was investigated using human tongue squamous cell c...
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2025-07-01
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| author | Merve Çiftçi Mansur Doğan Didem Duman Özge Göktuğ Temiz Mahmut Durmuş Efkan Bağda |
| author_facet | Merve Çiftçi Mansur Doğan Didem Duman Özge Göktuğ Temiz Mahmut Durmuş Efkan Bağda |
| author_sort | Merve Çiftçi |
| collection | DOAJ |
| description | <i>Background and Objectives</i>: The efficacy of newly synthesized water-soluble octa-mercaptopyridine-substituted oxotitanium (IV) phthalocyanine (oxo-TiPc) and copper (II) phthalocyanine (CuPc) compounds in photodynamic therapy (PDT) was investigated using human tongue squamous cell cancer cell line (SCC-9, ATCC) cultures. <i>Materials and Methods</i>: A laser light source with a wavelength of 635 nm was used for this study. The cytotoxic values of the cancerous (SCC-9) and healthy (L-929) cell samples to which different Pc concentrations were applied under laser light were evaluated spectroscopically with the XTT method. <i>Results</i>: The oxo-TiPc compound exhibited a significantly lower IC50 value (46.8 µM) for SCC-9 cells compared to the CuPc compound (286.2 µM), indicating higher anticancer activity. This cytotoxicity may be due to decreased aggregation and enhanced reactive oxygen species (ROS) formation. Double-staining tests confirmed that oxo-TiPc-induced cell death included both apoptosis and necrosis. <i>Conclusions</i>: The findings show that the oxo-TiPc compound, unlike the CuPc compound, exhibited more selective toxicity to the SCC-9 cell line and has a higher phototoxic effect. |
| format | Article |
| id | doaj-art-026e1af5055f42d1a7e47c2efaab8ca5 |
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| issn | 1010-660X 1648-9144 |
| language | English |
| publishDate | 2025-07-01 |
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| spelling | doaj-art-026e1af5055f42d1a7e47c2efaab8ca52025-07-25T13:29:38ZengMDPI AGMedicina1010-660X1648-91442025-07-01617128510.3390/medicina61071285In Vitro Efficacy of Water-Soluble Mercaptopyridine-Substituted Oxotitanium (IV) Phthalocyanine Compounds in Photodynamic Therapy of Oral Squamous Cell CarcinomaMerve Çiftçi0Mansur Doğan1Didem Duman2Özge Göktuğ Temiz3Mahmut Durmuş4Efkan Bağda5Division of Otorhinolaryngology, Tokat Erbaa State Hospital, 60500 Tokat, TürkiyeDepartment of Otorhinolaryngology, Faculty of Medicine, Sivas Cumhuriyet University, 58140 Sivas, TürkiyeDepartment of Molecular Biology and Genetics, Faculty of Science, Sivas Cumhuriyet University, 58140 Sivas, TürkiyeDepartment of Chemistry, Gebze Technical University, 41400 Gebze, TürkiyePharmaceutical Development Department of R&D Center, Abdi Ibrahim Pharmaceuticals, 34538 Istanbul, TürkiyeDepartment of Molecular Biology and Genetics, Faculty of Science, Sivas Cumhuriyet University, 58140 Sivas, Türkiye<i>Background and Objectives</i>: The efficacy of newly synthesized water-soluble octa-mercaptopyridine-substituted oxotitanium (IV) phthalocyanine (oxo-TiPc) and copper (II) phthalocyanine (CuPc) compounds in photodynamic therapy (PDT) was investigated using human tongue squamous cell cancer cell line (SCC-9, ATCC) cultures. <i>Materials and Methods</i>: A laser light source with a wavelength of 635 nm was used for this study. The cytotoxic values of the cancerous (SCC-9) and healthy (L-929) cell samples to which different Pc concentrations were applied under laser light were evaluated spectroscopically with the XTT method. <i>Results</i>: The oxo-TiPc compound exhibited a significantly lower IC50 value (46.8 µM) for SCC-9 cells compared to the CuPc compound (286.2 µM), indicating higher anticancer activity. This cytotoxicity may be due to decreased aggregation and enhanced reactive oxygen species (ROS) formation. Double-staining tests confirmed that oxo-TiPc-induced cell death included both apoptosis and necrosis. <i>Conclusions</i>: The findings show that the oxo-TiPc compound, unlike the CuPc compound, exhibited more selective toxicity to the SCC-9 cell line and has a higher phototoxic effect.https://www.mdpi.com/1648-9144/61/7/1285oxotitanium (IV) phthalocyanineSCC-9oral squamous cell carcinoma (OSCC)PDT |
| spellingShingle | Merve Çiftçi Mansur Doğan Didem Duman Özge Göktuğ Temiz Mahmut Durmuş Efkan Bağda In Vitro Efficacy of Water-Soluble Mercaptopyridine-Substituted Oxotitanium (IV) Phthalocyanine Compounds in Photodynamic Therapy of Oral Squamous Cell Carcinoma Medicina oxotitanium (IV) phthalocyanine SCC-9 oral squamous cell carcinoma (OSCC) PDT |
| title | In Vitro Efficacy of Water-Soluble Mercaptopyridine-Substituted Oxotitanium (IV) Phthalocyanine Compounds in Photodynamic Therapy of Oral Squamous Cell Carcinoma |
| title_full | In Vitro Efficacy of Water-Soluble Mercaptopyridine-Substituted Oxotitanium (IV) Phthalocyanine Compounds in Photodynamic Therapy of Oral Squamous Cell Carcinoma |
| title_fullStr | In Vitro Efficacy of Water-Soluble Mercaptopyridine-Substituted Oxotitanium (IV) Phthalocyanine Compounds in Photodynamic Therapy of Oral Squamous Cell Carcinoma |
| title_full_unstemmed | In Vitro Efficacy of Water-Soluble Mercaptopyridine-Substituted Oxotitanium (IV) Phthalocyanine Compounds in Photodynamic Therapy of Oral Squamous Cell Carcinoma |
| title_short | In Vitro Efficacy of Water-Soluble Mercaptopyridine-Substituted Oxotitanium (IV) Phthalocyanine Compounds in Photodynamic Therapy of Oral Squamous Cell Carcinoma |
| title_sort | in vitro efficacy of water soluble mercaptopyridine substituted oxotitanium iv phthalocyanine compounds in photodynamic therapy of oral squamous cell carcinoma |
| topic | oxotitanium (IV) phthalocyanine SCC-9 oral squamous cell carcinoma (OSCC) PDT |
| url | https://www.mdpi.com/1648-9144/61/7/1285 |
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