Cytotoxicity and biocompatibility of high mol% yttria containing zirconia
ObjectivesYttria-stabilized tetragonal phase zirconia has been used as a dental restorative material for over a decade. While it is still the strongest and toughest ceramic, its translucency remains as a significant drawback. To overcome this, stabilizing the translucency zirconia to a significant c...
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Korean Academy of Conservative Dentistry
2020-11-01
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| Series: | Restorative Dentistry & Endodontics |
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| Online Access: | http://rde.ac/upload/pdf/rde-45-e52.pdf |
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| author | Gulsan Ara Sathi Kazi Ryo Yamagiwa |
| author_facet | Gulsan Ara Sathi Kazi Ryo Yamagiwa |
| author_sort | Gulsan Ara Sathi Kazi |
| collection | DOAJ |
| description | ObjectivesYttria-stabilized tetragonal phase zirconia has been used as a dental restorative material for over a decade. While it is still the strongest and toughest ceramic, its translucency remains as a significant drawback. To overcome this, stabilizing the translucency zirconia to a significant cubic crystalline phase by increasing the yttria content to more than 8 mol% (8YTZP). However, the biocompatibility of a high amount of yttria is still an important topic that needs to be investigated.Materials and MethodsCommercially available 8YTZP plates were used. To enhance cell adhesion, proliferation, and differentiation, the surface of the 8YTZP is sequentially polished with a SiC-coated abrasive paper and surface coating with type I collagen. Fibroblast-like cells L929 used for cell adherence and cell proliferation analysis, and mouse bone marrow-derived mesenchymal stem cells (BMSC) used for cell differentiation analysis.ResultsThe results revealed that all samples, regardless of the surface treatment, are hydrophilic and showed a strong affinity for water. Even the cell culture results indicate that simple surface polishing and coating can affect cellular behavior by enhancing cell adhesion and proliferation. Both L929 cells and BMSC were nicely adhered to and proliferated in all conditions.ConclusionsThe results demonstrate the biocompatibility of the cubic phase zirconia with 8 mol% yttria and suggest that yttria with a higher zirconia content are not toxic to the cells, support a strong adhesion of cells on their surfaces, and promote cell proliferation and differentiation. All these confirm its potential use in tissue engineering. |
| format | Article |
| id | doaj-art-01fe86f5fdbb4b5db93e324fd2a47d9d |
| institution | Matheson Library |
| issn | 2234-7658 2234-7666 |
| language | English |
| publishDate | 2020-11-01 |
| publisher | Korean Academy of Conservative Dentistry |
| record_format | Article |
| series | Restorative Dentistry & Endodontics |
| spelling | doaj-art-01fe86f5fdbb4b5db93e324fd2a47d9d2025-07-03T04:14:34ZengKorean Academy of Conservative DentistryRestorative Dentistry & Endodontics2234-76582234-76662020-11-01454e5210.5395/rde.2020.45.e52937Cytotoxicity and biocompatibility of high mol% yttria containing zirconiaGulsan Ara Sathi Kazi0Ryo Yamagiwa1Department of Biosystems Engineering, Graduate School of Science and Technology, Yamagata University, Yamagata, JapanDepartment of Biosystems Engineering, Graduate School of Science and Technology, Yamagata University, Yamagata, JapanObjectivesYttria-stabilized tetragonal phase zirconia has been used as a dental restorative material for over a decade. While it is still the strongest and toughest ceramic, its translucency remains as a significant drawback. To overcome this, stabilizing the translucency zirconia to a significant cubic crystalline phase by increasing the yttria content to more than 8 mol% (8YTZP). However, the biocompatibility of a high amount of yttria is still an important topic that needs to be investigated.Materials and MethodsCommercially available 8YTZP plates were used. To enhance cell adhesion, proliferation, and differentiation, the surface of the 8YTZP is sequentially polished with a SiC-coated abrasive paper and surface coating with type I collagen. Fibroblast-like cells L929 used for cell adherence and cell proliferation analysis, and mouse bone marrow-derived mesenchymal stem cells (BMSC) used for cell differentiation analysis.ResultsThe results revealed that all samples, regardless of the surface treatment, are hydrophilic and showed a strong affinity for water. Even the cell culture results indicate that simple surface polishing and coating can affect cellular behavior by enhancing cell adhesion and proliferation. Both L929 cells and BMSC were nicely adhered to and proliferated in all conditions.ConclusionsThe results demonstrate the biocompatibility of the cubic phase zirconia with 8 mol% yttria and suggest that yttria with a higher zirconia content are not toxic to the cells, support a strong adhesion of cells on their surfaces, and promote cell proliferation and differentiation. All these confirm its potential use in tissue engineering.http://rde.ac/upload/pdf/rde-45-e52.pdfzirconiayttriabiocompatibilitycellular activity |
| spellingShingle | Gulsan Ara Sathi Kazi Ryo Yamagiwa Cytotoxicity and biocompatibility of high mol% yttria containing zirconia Restorative Dentistry & Endodontics zirconia yttria biocompatibility cellular activity |
| title | Cytotoxicity and biocompatibility of high mol% yttria containing zirconia |
| title_full | Cytotoxicity and biocompatibility of high mol% yttria containing zirconia |
| title_fullStr | Cytotoxicity and biocompatibility of high mol% yttria containing zirconia |
| title_full_unstemmed | Cytotoxicity and biocompatibility of high mol% yttria containing zirconia |
| title_short | Cytotoxicity and biocompatibility of high mol% yttria containing zirconia |
| title_sort | cytotoxicity and biocompatibility of high mol yttria containing zirconia |
| topic | zirconia yttria biocompatibility cellular activity |
| url | http://rde.ac/upload/pdf/rde-45-e52.pdf |
| work_keys_str_mv | AT gulsanarasathikazi cytotoxicityandbiocompatibilityofhighmolyttriacontainingzirconia AT ryoyamagiwa cytotoxicityandbiocompatibilityofhighmolyttriacontainingzirconia |