Orai1 Expression and Vascular Function in Kidney Donors Determine Graft Outcomes at Short/Mid-Term
We aimed to determine the influence of donors’ vascular function on renal function in recipients and to evaluate the role of Orai1 calcium channels as a potential marker. A prospective collaborative multicenter study was designed. Blood, aorta (HA), mesenteric arteries (HMAs) and corpus cavernosum (...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-07-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/14/13/1005 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1839632038967115776 |
|---|---|
| author | Esther García-Rojo Javier Angulo Mariam El Assar Rocío Santos-Pérez de la Blanca Borja García-Gómez José Medina-Polo Alejandro Sevilleja-Ortiz Leocadio Rodríguez-Mañas Argentina Fernández Eduardo Gutiérrez-Martínez Enrique Morales-Ruiz Alfredo Rodríguez-Antolín Javier Romero-Otero |
| author_facet | Esther García-Rojo Javier Angulo Mariam El Assar Rocío Santos-Pérez de la Blanca Borja García-Gómez José Medina-Polo Alejandro Sevilleja-Ortiz Leocadio Rodríguez-Mañas Argentina Fernández Eduardo Gutiérrez-Martínez Enrique Morales-Ruiz Alfredo Rodríguez-Antolín Javier Romero-Otero |
| author_sort | Esther García-Rojo |
| collection | DOAJ |
| description | We aimed to determine the influence of donors’ vascular function on renal function in recipients and to evaluate the role of Orai1 calcium channels as a potential marker. A prospective collaborative multicenter study was designed. Blood, aorta (HA), mesenteric arteries (HMAs) and corpus cavernosum (HCC) specimens were obtained from organ donors at the kidney procurement procedure (n = 60). Evolution (up to 2 years) of renal function measured as serum creatinine (SCr) and glomerular filtration rate (GFR) was evaluated in respective recipients (n = 64). Vascular responses were determined in HA, HMA and HCC from donors. Tumor necrosis factor-α, asymmetric dimethyl arginine and Orai1 were measured in plasma. Orai1 protein expression was also evaluated in each donor’s aorta. Endothelium-dependent vasodilation (HMA, HCC) and adrenergic contraction (HA) in donors determined renal function in recipients, 12 months post-transplantation. Donors in the best quartile of vascular function predicted lower SCr and higher GFR in kidney recipients for 12/24 months. Plasma Orai1 in donors was negatively correlated with vascular function and predicted renal function at 3–6 months post-transplantation. Donor Orai1 vascular content was associated with reduced vascular function and with poorer recipient renal function for 1-year post-transplantation. Systemic vascular function of kidney donors determines recipients’ renal function at short/mid-term. Donors’ vascular function and recipients’ renal function are negatively associated with donors’ Orai1 vascular expression, being a potential biomarker of renal outcomes. |
| format | Article |
| id | doaj-art-01b06b0624e24e7d810f11fd098a477a |
| institution | Matheson Library |
| issn | 2073-4409 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj-art-01b06b0624e24e7d810f11fd098a477a2025-07-11T14:37:53ZengMDPI AGCells2073-44092025-07-011413100510.3390/cells14131005Orai1 Expression and Vascular Function in Kidney Donors Determine Graft Outcomes at Short/Mid-TermEsther García-Rojo0Javier Angulo1Mariam El Assar2Rocío Santos-Pérez de la Blanca3Borja García-Gómez4José Medina-Polo5Alejandro Sevilleja-Ortiz6Leocadio Rodríguez-Mañas7Argentina Fernández8Eduardo Gutiérrez-Martínez9Enrique Morales-Ruiz10Alfredo Rodríguez-Antolín11Javier Romero-Otero12Department of Urology, Hospital Universitario HM Sanchinarro, HM Hospitales, 28050 Madrid, SpainDepartment of Histology-Research, Unidad de Investigación Traslacional en Cardiología (IRYCIS-UFV), Hospital Universitario Ramón y Cajal, 28034 Madrid, SpainCentro de Investigación Biomédica en Red sobre Fragilidad y Envejecimiento Saludable (CIBERFES), Instituto de Salud Carlos III, 28029 Madrid, SpainDepartment of Urology, Hospital Universitario 12 de Octubre, Instituto de Investigación Sanitaria Hospital 12 de Octubre (imas12), 28041 Madrid, SpainDepartment of Urology, Hospital Universitario 12 de Octubre, Instituto de Investigación Sanitaria Hospital 12 de Octubre (imas12), 28041 Madrid, SpainDepartment of Urology, Hospital Universitario 12 de Octubre, Instituto de Investigación Sanitaria Hospital 12 de Octubre (imas12), 28041 Madrid, SpainDepartment of Histology-Research, Unidad de Investigación Traslacional en Cardiología (IRYCIS-UFV), Hospital Universitario Ramón y Cajal, 28034 Madrid, SpainCentro de Investigación Biomédica en Red sobre Fragilidad y Envejecimiento Saludable (CIBERFES), Instituto de Salud Carlos III, 28029 Madrid, SpainDepartment of Histology-Research, Unidad de Investigación Traslacional en Cardiología (IRYCIS-UFV), Hospital Universitario Ramón y Cajal, 28034 Madrid, SpainDepartment of Nephrology, Hospital Universitario 12 de Octubre, Instituto de Investigación Sanitaria Hospital 12 de Octubre (imas12), 28041 Madrid, SpainDepartment of Nephrology, Hospital Universitario 12 de Octubre, Instituto de Investigación Sanitaria Hospital 12 de Octubre (imas12), 28041 Madrid, SpainDepartment of Urology, Hospital Universitario 12 de Octubre, Instituto de Investigación Sanitaria Hospital 12 de Octubre (imas12), 28041 Madrid, SpainDepartment of Urology, Hospital Universitario HM Sanchinarro, HM Hospitales, 28050 Madrid, SpainWe aimed to determine the influence of donors’ vascular function on renal function in recipients and to evaluate the role of Orai1 calcium channels as a potential marker. A prospective collaborative multicenter study was designed. Blood, aorta (HA), mesenteric arteries (HMAs) and corpus cavernosum (HCC) specimens were obtained from organ donors at the kidney procurement procedure (n = 60). Evolution (up to 2 years) of renal function measured as serum creatinine (SCr) and glomerular filtration rate (GFR) was evaluated in respective recipients (n = 64). Vascular responses were determined in HA, HMA and HCC from donors. Tumor necrosis factor-α, asymmetric dimethyl arginine and Orai1 were measured in plasma. Orai1 protein expression was also evaluated in each donor’s aorta. Endothelium-dependent vasodilation (HMA, HCC) and adrenergic contraction (HA) in donors determined renal function in recipients, 12 months post-transplantation. Donors in the best quartile of vascular function predicted lower SCr and higher GFR in kidney recipients for 12/24 months. Plasma Orai1 in donors was negatively correlated with vascular function and predicted renal function at 3–6 months post-transplantation. Donor Orai1 vascular content was associated with reduced vascular function and with poorer recipient renal function for 1-year post-transplantation. Systemic vascular function of kidney donors determines recipients’ renal function at short/mid-term. Donors’ vascular function and recipients’ renal function are negatively associated with donors’ Orai1 vascular expression, being a potential biomarker of renal outcomes.https://www.mdpi.com/2073-4409/14/13/1005kidney transplantationrenal functionOrai calcium channelsendothelial vasodilationadrenergic vasoconstrictionhuman mesenteric artery |
| spellingShingle | Esther García-Rojo Javier Angulo Mariam El Assar Rocío Santos-Pérez de la Blanca Borja García-Gómez José Medina-Polo Alejandro Sevilleja-Ortiz Leocadio Rodríguez-Mañas Argentina Fernández Eduardo Gutiérrez-Martínez Enrique Morales-Ruiz Alfredo Rodríguez-Antolín Javier Romero-Otero Orai1 Expression and Vascular Function in Kidney Donors Determine Graft Outcomes at Short/Mid-Term Cells kidney transplantation renal function Orai calcium channels endothelial vasodilation adrenergic vasoconstriction human mesenteric artery |
| title | Orai1 Expression and Vascular Function in Kidney Donors Determine Graft Outcomes at Short/Mid-Term |
| title_full | Orai1 Expression and Vascular Function in Kidney Donors Determine Graft Outcomes at Short/Mid-Term |
| title_fullStr | Orai1 Expression and Vascular Function in Kidney Donors Determine Graft Outcomes at Short/Mid-Term |
| title_full_unstemmed | Orai1 Expression and Vascular Function in Kidney Donors Determine Graft Outcomes at Short/Mid-Term |
| title_short | Orai1 Expression and Vascular Function in Kidney Donors Determine Graft Outcomes at Short/Mid-Term |
| title_sort | orai1 expression and vascular function in kidney donors determine graft outcomes at short mid term |
| topic | kidney transplantation renal function Orai calcium channels endothelial vasodilation adrenergic vasoconstriction human mesenteric artery |
| url | https://www.mdpi.com/2073-4409/14/13/1005 |
| work_keys_str_mv | AT esthergarciarojo orai1expressionandvascularfunctioninkidneydonorsdeterminegraftoutcomesatshortmidterm AT javierangulo orai1expressionandvascularfunctioninkidneydonorsdeterminegraftoutcomesatshortmidterm AT mariamelassar orai1expressionandvascularfunctioninkidneydonorsdeterminegraftoutcomesatshortmidterm AT rociosantosperezdelablanca orai1expressionandvascularfunctioninkidneydonorsdeterminegraftoutcomesatshortmidterm AT borjagarciagomez orai1expressionandvascularfunctioninkidneydonorsdeterminegraftoutcomesatshortmidterm AT josemedinapolo orai1expressionandvascularfunctioninkidneydonorsdeterminegraftoutcomesatshortmidterm AT alejandrosevillejaortiz orai1expressionandvascularfunctioninkidneydonorsdeterminegraftoutcomesatshortmidterm AT leocadiorodriguezmanas orai1expressionandvascularfunctioninkidneydonorsdeterminegraftoutcomesatshortmidterm AT argentinafernandez orai1expressionandvascularfunctioninkidneydonorsdeterminegraftoutcomesatshortmidterm AT eduardogutierrezmartinez orai1expressionandvascularfunctioninkidneydonorsdeterminegraftoutcomesatshortmidterm AT enriquemoralesruiz orai1expressionandvascularfunctioninkidneydonorsdeterminegraftoutcomesatshortmidterm AT alfredorodriguezantolin orai1expressionandvascularfunctioninkidneydonorsdeterminegraftoutcomesatshortmidterm AT javierromerootero orai1expressionandvascularfunctioninkidneydonorsdeterminegraftoutcomesatshortmidterm |