Orai1 Expression and Vascular Function in Kidney Donors Determine Graft Outcomes at Short/Mid-Term

Orai1 Expression and Vascular Function in Kidney Donors Determine Graft Outcomes at Short/Mid-Term

We aimed to determine the influence of donors’ vascular function on renal function in recipients and to evaluate the role of Orai1 calcium channels as a potential marker. A prospective collaborative multicenter study was designed. Blood, aorta (HA), mesenteric arteries (HMAs) and corpus cavernosum (...

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Main Authors: Esther García-Rojo, Javier Angulo, Mariam El Assar, Rocío Santos-Pérez de la Blanca, Borja García-Gómez, José Medina-Polo, Alejandro Sevilleja-Ortiz, Leocadio Rodríguez-Mañas, Argentina Fernández, Eduardo Gutiérrez-Martínez, Enrique Morales-Ruiz, Alfredo Rodríguez-Antolín, Javier Romero-Otero
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Cells
Subjects:
kidney transplantation
renal function
Orai calcium channels
endothelial vasodilation
adrenergic vasoconstriction
human mesenteric artery
Online Access:https://www.mdpi.com/2073-4409/14/13/1005
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Summary:We aimed to determine the influence of donors’ vascular function on renal function in recipients and to evaluate the role of Orai1 calcium channels as a potential marker. A prospective collaborative multicenter study was designed. Blood, aorta (HA), mesenteric arteries (HMAs) and corpus cavernosum (HCC) specimens were obtained from organ donors at the kidney procurement procedure (n = 60). Evolution (up to 2 years) of renal function measured as serum creatinine (SCr) and glomerular filtration rate (GFR) was evaluated in respective recipients (n = 64). Vascular responses were determined in HA, HMA and HCC from donors. Tumor necrosis factor-α, asymmetric dimethyl arginine and Orai1 were measured in plasma. Orai1 protein expression was also evaluated in each donor’s aorta. Endothelium-dependent vasodilation (HMA, HCC) and adrenergic contraction (HA) in donors determined renal function in recipients, 12 months post-transplantation. Donors in the best quartile of vascular function predicted lower SCr and higher GFR in kidney recipients for 12/24 months. Plasma Orai1 in donors was negatively correlated with vascular function and predicted renal function at 3–6 months post-transplantation. Donor Orai1 vascular content was associated with reduced vascular function and with poorer recipient renal function for 1-year post-transplantation. Systemic vascular function of kidney donors determines recipients’ renal function at short/mid-term. Donors’ vascular function and recipients’ renal function are negatively associated with donors’ Orai1 vascular expression, being a potential biomarker of renal outcomes.
ISSN:2073-4409

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