Search Results - ZHANG Xiao-dong
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Construction of cell line stably expressing IBDV VP 2 protein mutants
Published 2011-09-01Get full textA couple of primers was designed on the base of VP2 gene sequence of infectious bursal disease virus (IBDV) HZ2 strain, and the VP2 gene was successfully cloned into eukaryotic expression vector pDsRed2-N1. Then, the recombinant plasmid pDsRed2-N1-VP2 was constructed. The 279 site and 284 site on VP...
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Pathogenicity and immunogenicity of recombinant methyltransferase-defective vesicular stomatitis virus
Published 2011-07-01Get full textTo prevent the vesicular stomatitis virus (VSV) disease, using a panel of recombinant VSV (E1764A, S1827A, Y1650A, and F1691A) that were defective in mRNA cap methylation as vaccine to inoculate five-week-old BALB/c female mice through oral route with 1×10<sup>6</sup> PFU, the pathogenic...
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Construction of plasmids containing IBDV VP2/4/3 and ChIL-18 fusion genes and co-expression genes as well as proteins expressed on Vero cells
Published 2010-01-01Get full textIn order to improve the immunity effect of infectious bursal disease virus (IBDV) polyprotein (VP2/4/3) DNA vaccine, fusion genes of IBDV polyprotein (VP2/4/3) gene and chicken interleukin 18 (ChIL-18) gene were constructed using the technique of splicing by overlapping extension (SOEing) and three...
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Establishment of infectious bursal disease virus (IBDV) reverse genetics system using T 7 RNA polymerase
Published 2008-11-01Get full textIn order to establish the reverse genetics of IBDV, the sequences of T 7 promoter and ribozyme HDV were introduced into the 5 and 3 terminals of segments A and B of IBDV genome using PCR. Then the segments, which containing promoter and ribozyme HDV sequences, were cloned into PT-Pluc vector and nam...
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Immunoenhancement on DNA vaccine of polyprotein gene in infectious bursal disease virus by co-delivery with plasmid encoding chicken interleukin-18
Published 2008-01-01Get full textTo further evaluate the immunoenhancement of chicken interleukin-18 (IL-18), the polyprotein of infectious bursal disease virus (IBDV) was prepared to DNA vaccine, and the DNA vaccine was coadministrated with the eukaryotic expression plasmid encoding interleukin-18 (pCI-IL-18), then boosted after 2...
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Comparative Study of Corrosion Rates Determined by Several Methods for Q235 Steel
Published 2021-02-01Get full textElectrochemical measurement and mass loss have been applied to acquire the corrosion rate, but the accuracy is limited when the corrosion is in its early stages. In this study, the early stages corrosion behavior of Q235 steel in 3.5% NaCl solution with pH=5 was investigated. The early stages corros...
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Why Anti-Energetic Agents Such as Citrate or 3-Bromopyruvate Should be Tested as Anti-Cancer Agents: Experimental In Vitro and In Vivo Studies
Published 2012Get full textNone
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