Search Results - Ya Feng
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Molecular characterization of Apple necrotic mosaic virus identified in crabapple (Malus spp.) tree of China
Published 2019-03-01Get full textApple necrotic mosaic virus (ApNMV) was identified in crabapple trees with mosaic symptoms from Zaozhuang, Shandong Province, China, by reverse transcription polymerase chain reaction (RT-PCR) analysis. The complete nucleotide sequences of one isolate from crabapple (ApNMV-Hai) and two isolates from...
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Effect of pre-culture on virus elimination from in vitro apple by thermotherapy coupled with shoot tip culture
Published 2018-09-01Get full textWe evaluated the role of pre-culture on survival rate of in vitro apple plants treated by thermotherapy. Two apple cultivars, Malus×domestica cv. Pink Lady and Huafu, were used in the experiment and both have widely grown in China and infected with Apple chlorotic leaf spot virus (ACLSV) and Apple s...
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Prevalence and genetic diversity of grapevine fabavirus isolates from different grapevine cultivars and regions in China
Published 2020-03-01Get full textA total of 288 grapevine samples of 61 different grapevine cultivars, collected from 22 provinces and regions, were analyzed by reverse transcription-PCR (RT-PCR) for the presence of grapevine fabavirus (GFabV). PCR detection results showed the incidences of GFabV were 12.8% (30/235) and 48.1% (25/5...
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A sensitive SYBR Green RT-qPCR method for grapevine virus E and its application for virus detection in different grapevine sample types
Published 2020-07-01Get full textTo develop a rapid and high-sensitivity method for detection of grapevine virus E (GVE), a SYBR Green based real-time fluorescence quantitative RT-PCR method (RT-qPCR) was established. This method could be used to detect GVE specifically, and the sensitivity was about 100 times greater than conventi...
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Effectiveness and Cost-Effectiveness of a Mobile Psychoeducation Program (OkeyMind) for Social Anxiety Symptoms Among Youths: Protocol for a Randomized Controlled Trial
Published 2025-07-01Get full textBackgroundSocial anxiety is increasingly prevalent among youths, leading to social withdrawal, isolation, and heightened depression risk. Mobile health (mHealth) interventions offer anonymity, accessibility, and personalized support, but their effectiveness and cost-effective...
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Bufei Jiedu Formula enhances CD40 activation and macrophage polarization to eliminate intracellular MRSA persisters
Published 2025-07-01Get full textBackgroundIntracellular methicillin-resistant Staphylococcus aureus (MRSA) represents a complex infection in clinical practice, characterized by its refractory and recurrent nature, rendering it challenging to treat with conventional antibiotics. Bufei Jiedu Formula (BFJD) is a traditional Chinese m...
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