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Highly efficient generation of GGTA1 knockout pigs using a combination of TALEN mRNA and magnetic beads with somatic cell nuclear transfer by Chong FENG, Xi-rui LI, Hui-ting CUI, Chuan LONG, Xia LIU, Xing-hua TIAN, Deng-ke PAN, Yu-zhu LUO
Published 2016-07-01The transcription activator-like effector nuclease (TALEN) technique combined with the somatic cell nuclear transfer (SCNT) method has been successfully applied for creating genetically modified pigs. However, methods for isolating cells with biallelic indels requires further improvement because of...
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