Search Results - Siegfried Roth

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    Egg tanning improves the efficiency of CRISPR/Cas9-mediated mutant locust production by enhancing defense ability after microinjection

    Egg tanning improves the efficiency of CRISPR/Cas9-mediated mutant locust production by enhancing defense ability after microinjection by Ting-ting ZHANG, Ting-mei WEN, Yang YUE, Qiang YAN, Er-xia DU, San-hong FAN, Siegfried ROTH, Sheng LI, Jian-zhen ZHANG, Xue-yao ZHANG, Min ZHANG

    Published 2021-10-01

    The mutant efficiency and hatching ratio are two key factors that significantly affect the construction of genome-modified mutant insects. In the construction of CRISPR/Cas9-mediated dsLmRNase2–/– mutant locusts, we found that the tanned eggs which experienced a 20-min contact with the oocyst exhibi...

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    CRISPR/Cas9-Mediated Knockout of the <i>Corazonin</i> Gene Indicates Its Regulation on the Cuticle Development of Desert Locusts (<i>Schistocerca gregaria</i>)

    CRISPR/Cas9-Mediated Knockout of the <i>Corazonin</i> Gene Indicates Its Regulation on the Cuticle Development of Desert Locusts (<i>Schistocerca gregaria</i>) by Yingying He, Qiang Yan, Yong Bi, Guosheng Liu, Shuang Hou, Xinyi Chen, Xiaoming Zhao, Xueyao Zhang, Min Zhang, Jianzhen Zhang, Binbin Ma, Benjamin Warren, Siegfried Roth, Tingting Zhang

    Published 2025-07-01

    The desert locust (<i>Schistocerca gregaria</i>) represents one of the most destructive agricultural pests globally, renowned for its ability to form massive swarms that can devastate crops and threaten food security across vast regions. Despite the widespread application of the CRISPR/C...

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