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    A novel real-time RT-PCR with TaqMan-MGB probes and its application in detecting BVDV infections in dairy farms

    A novel real-time RT-PCR with TaqMan-MGB probes and its application in detecting BVDV infections in dairy farms by Yong-qiang ZHANG, Hai-sheng LIU, Xiao-dong WU, Xiao-zhen WANG, Jin-ming LI, Yong-gang ZHAO, Yan LÜ, Wei-jie REN, Sheng-qiang GE, Zhi-liang WANG

    Published 2015-08-01

    A real-time RT-PCR assay using TaqMan-MGB probes was developed to detect and type the bovine viral diarrhea virus (BVDV) in cattle. Universal primers and TaqMan-MGB probes were designed from the 5′-untranslated region of known pestiviral sequences. Prior to optimizing the assay, cRNAs were transcrib...

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