Ngā hua rapu - Qing YAO

The lethal and sub-lethal effects of a transgenic rice line KMD1, containing a synthetic cry1Ab gene from Bacillus thuringiensis measured on larvae of striped stem borer, Chilo suppressalis (Walker) is reported in this article. The transgenic rice line was highly toxic to the 1st, 3rd and 5th instar...

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Growth inhibition of Agrobacterium by 3 different antibiotics was demonstrated. Carbenicillin and cefotaxime gave better results than ampicillin in inhibiting Agrobacterium growth. Cefotaxime was significantly more toxic than carbenicillin in induction of callus in sorghum. Carbenicillin of concentr...

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A survey of the population densities of rice planthoppers is important for forecasting decisions and efficient control. Traditional manual surveying of rice planthoppers is time-consuming, fatiguing, and subjective. A new three-layer detection method was proposed to detect and identify white-backed...

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The time-consuming in vitro bioassay is the frequently used method to screen insect resistant transgenic rice and often remains a tedious task for us. Here we reported the resistance of Bt transgenic rice to striped stem borer by means of field natural infestation and in vitro bioassay. We found tha...

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With Milyang 46 (MY46) taken as recurrent line and a transgenic Bt rice (KMD1) as donor line, an isogenic line with Bt gene (RIMY46) was constructed by consecutive backcrosses and selections with GUS reaction as a marker. It was found that GUS reaction and PCR amplification test for Bt gene were pos...

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Experimental study of the inheritance of fertility restoration of cytoplasmic male sterility (CMS)in rice was undertaken, using three test-cross populations of Xieqingzao A/ (Xieqingzao B/Milyang 46)F₆, Xieqingzao A/ (Zhenshan 97 B/Milyang 46)F₆ and Zhen...

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The effects of factors including seedling age, different combinations of BA and NAA concentrations, Agar concentration, AgNO₃ and GA₃ on bud induction from cotyledon are analysed. The results indicate that the explants from 5-9 days old seedling are the...

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Recombinant inbred lines population derived from Zhenshan 97B/Miyang 46 (ZS97B/MY46) and its genetic linkage map were used to map the QTLs controlling rice transparency in different environments, Hainan and Hangzhou. A total of five QTLs with genetic main effects were detected on chromosomes 2, 6 (t...

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Based on our early rice breeding practices in Hangzhou and in South China region, 14 varieties (or pure lines) with different apparent amylose content (AAC) were planted in early and late season in Hangzhou (30° N, HZE, HZL), Zhejiang province, East China, and winter season in Hainan province, South...

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Using the Bt rice “KMD” as the insect-resistant donor, three transgenic Japonica rice lines with high yield and insect resistance were successfully developed from crosses between the Bt rice and Japonica rice variety after pedigree selections lasting eight generations through GUS tissue staining-aid...

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Cruciferous vegetables are important edible vegetable crops. However, they are susceptible to various pests during their growth process, which requires real-time and accurate monitoring of these pests for pest forecasting and scientific control. Hanging yellow sticky boards is a common way to monito...

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A quantitative survey of rice planthoppers in paddy fields is important to assess the population density and make forecasting decisions. Manual rice planthopper survey methods in paddy fields are time-consuming, fatiguing and tedious. This paper describes a handheld device for easily capturing plant...

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Nitrogen (N) and potassium (K) are two key mineral nutrient elements involved in rice growth. Accurate diagnosis of N and K status is very important for the rational application of fertilizers at a specific rice growth stage. Therefore, we propose a hybrid model for diagnosing rice nutrient levels a...

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Identification and counting of rice light-trap pests are important to monitor rice pest population dynamics and make pest forecast. Identification and counting of rice light-trap pests manually is time-consuming, and leads to fatigue and an increase in the error rate. A rice light-trap insect imagin...

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Oomycete, particularly Phytophthora species, causes the most devastating crop diseases, such as potato late blight, and threatens the sustainable crop production worldwide. Our previous studies identified Resistance to Phytophthora parasitica 1 (RTP1) as a negative regulator of Arabidopsis resistanc...

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IntroductionActinobacillus pleuropneumoniae is an important respiratory pathogen, which can cause porcine contagious pleuropneumonia and lead to great economic losses to worldwide swine industry. High potassium is an adverse environment for bacteria, which is not conducive to providing turgor pressu...

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Instability of silk fibroin nanoparticles (SFNPs) in physiologic condition hinders its application as drug delivery vehicle. Herein, indocyanine green (ICG) loaded silk fibroin nanoparticles (ICG-SFNPs) was firstly prepared and then crosslinked by proanthocyanidins to obtain the stable ICG-CSFNPs fo...

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Monitoring pest populations in paddy fields is important to effectively implement integrated pest management. Light traps are widely used to monitor field pests all over the world. Most conventional light traps still involve manual identification of target pests from lots of trapped insects, which i...

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Objective: The cyclic GMP-AMP synthase (cGAS)-stimulator of interferon (IFN) genes (STING) signaling pathway has been implicated in the initiation and maintenance of a variety of inflammatory diseases. Thus, the search for modulators of the cGAS-STING signaling pathway is likely to contribute to the...

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