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    Nested PCR amplification of the mitochondrial hypervariable region for non-invasive eDNA detection of Cryptobranchus alleganiensis. by Patrick Kurtz, Jorge Santo Domingo, Robert Alexander Pyron, David Wendell

    Published 2025-01-01

    Environmental DNA (eDNA) has proven an invaluable tool in detecting elusive, endangered, or otherwise hard-to-identify species in aquatic ecosystems. The Eastern hellbender salamander (Cryptobranchus alleganiensis) is a fully aquatic amphibian with an increasingly threatened population due to its re...

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