Kết quả tìm kiếm - N. A. Sharapova
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1
Modern Methods of DNA Sequencing (Scientific Review) Bằng Ya. M. Krasnov, N. P. Guseva, N. A. Sharapova, A. V. Cherkasov
Được phát hành 2014-06-01Rapidly developing sequencing technologies have provided the possibility for identification of a DNA nucleotide sequence of a whole individual human genome just in a couple of weeks. Working capacity of some sequencers is already measured in thousands of milliards of base pairs per an operating cycl...
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2
Determination of the Activity of an Experimental Anti-choleric Immunoenterosorbent <i>in Vitro</i> in Dot-immunoanalysis Bằng M. V. Ovchinnikova, E. G. Abramova, M. N. Kireev, T. Yu. Kirillova, N. A. Sharapova, V. V. Rogozhin
Được phát hành 2022-05-01Introduction. At the rate of World health organization from 1 to 4 million people annually get infected with cholera, and around 143 000 people become a victim of this infection. At this point of seventh pandemic WHO and its partners actively evaluate new methods of treatment and prevention of chole...
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3
Development of the PCR Assay with Universal Primers for the Detection of Different <i>tcpA</i> Gene Variants Bằng A. V. Fadeeva, G. A. Eroshenko, G. N. Odinokov, N. A. Sharapova, T. V. Valova, V. V. Kutyrev
Được phát hành 2012-02-01Developed is the PCR assay for the detection of the structural genes of toxin co-regulated adhesion piluses - tcpA of different types. Determined are the universal primers, the usage of which provides for the detection of the stated above genes in V. cholerae of various serogroups. With the help of...
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4
THE INFLUENCE OF COLLOID SILVER NANO-PARTICLES UPON IMMUNOBIOLOGIC PROPERTIES OF PLAGUE MICROBE CAPSULAR ANTIGEN Bằng M. N. Kireyev, N. P. Gooseva, T. A. Polunina, A. L. Kravtsov, S. A. Bugorkova, N. A. Sharapova, T. M. Taranenko
Được phát hành 2012-09-01This study shows that Yersinia pestis capsular antigen F1 conjugated to the CS nano-particles demonstrates apparent immunogenic effects. When injected to the test animals it was able to stimulate the production of specific antibodies under smaller antigenic load, it caused activation of the genetic...
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5
Taxonomic Position of Entomoparasitic Nematodes Isolated from Fleas in Gorno-Altai High-Mountain Natural Plague Focus Bằng E. G. Oglodin, E. G. Tokmakova, K. A. Nikiforov, A. V. Denisov, N. A. Sharapova, G. A. Eroshenko
Được phát hành 2018-07-01Objective of the study was to investigate entomoparasitic nematodes from rodent fleas, collected in Gorno-Altai high-mountain natural plague focus in 2016–2017, with identification of their taxonomic status. Materials and methods. Given are the results of morphological and genetic analysis of entomo...
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6
Effect of <i>Yersinia pestis</i> on the Soil Nematodes <i>Panagrolaimus</i> sp. from the Gorno-Altai High-Mountain Focus of Plague Bằng M. A. Makashova, E. G. Oglodin, N. A. Sharapova, A. E. Samoilov, G. A. Eroshenko, V. V. Kutyrev
Được phát hành 2023-07-01The aim of the work was to study interaction of Yersinia pestis with soil nematodes isolated on the territory of the Gorno-Altai high-mountain plague focus. Materials and methods. We used the fluorescent Y. pestis strain KM2083, a derivative of the natural strain of the 4.ANT phylogenetic line, the...
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7
Extensive Studies of Legionella Strains Isolated from the Environmental Objects during Preparation and Holding of Mass Events in the Russian Federation in 2013-2014 Bằng S. A. Portenko, A. S. Abdrashitova, N. E. Shcherbakova, P. S. Erokhin, Ya. M. Krasnov, N. P. Guseva, N. A. Sharapova, S. A. Shcherbakova, V. V. Kutyrev
Được phát hành 2020-01-01Objective of the work was to conduct an extended study of Legionella strains isolated from epidemiologically significant environmental objects during the preparation and conduct of mass events in the territory of the Russian Federation in 2013-2014 . Materials and methods. Studied were 53 strains of...
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8
Long-Term Persistence of <i>Yersinia pestis</i> in Association with Acanthamoeba castellanii in Experiment Bằng M. A. Makashova, E. G. Oglodin, L. M. Kukleva, N. A. Sharapova, E. A. Naryshkina, V. G. Germanchuk, G. A. Eroshenko, V. V. Kutyrev
Được phát hành 2023-02-01The aim of the study was to test the feasibility of long-term survival and preservation of the properties of Yersinia pestis in association with soil amoeba Acanthamoeba castellanii. Materials and methods. Y. pestis strains and acanthamoeba isolated in the common area of the Gorno-Altai high-mountai...
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9
Determination of the Activity of the Anti-Rabies Sera and Heterologous Anti-Rabies Immunoglobulin <i>in vitro</i> in the Dot Immunoassay Bằng N. A. Sharapova, E. G. Abramova, A. K. Nikiforov, M. N. Kireev, L. V. Savitskaya, L. N. Minaeva, T. A. Mikheeva, M. V. Galkina, Ya. M. Krasnov
Được phát hành 2010-02-01For the first time constructed was a diagnosticum based on colloid gold nanoparticles (with an average size of about 15-17 nm) to detect specific antibodies against rabies virus in the anti-rabies sera and immunoglobulin in vitro in the dot-immunoassay [24]. Investigated was the correlation between...
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10
Occurrence of Genetic Determinants Associated with Attenuation in Wild-Type Strains of the Tularemia Microbe Bằng E. A. Naryshkina, N. A. Osina, S. D. Katyshev, Ya. M. Krasnov, N. A. Sharapova, A. V. Fedorov, A. D. Katyshev, O. Yu. Lyashova, A. V. Osin, Z. L. Devdariani, S. A. Shcherbakova
Được phát hành 2025-07-01The aim of our work was to determine genetic markers associated with attenuation (deletions in RD-18, RD-19 positions, single mutations) in natural strains of the causative agent of tularemia. Materials and methods. The work used 107 Francisella tularensis strains from the State Collection of Pathog...
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11
OPTIMIZATION OF PRESENTATION AND CONSUMER CONTAINER OF ANTI-RABIES IMMUNOGLOBULIN OBTAINED FROM HORSE SERUM Bằng N. N. Kochkalova, E. G. Abramova, A. K. Nikiforov, M. N. Kireyev, O. A. Lobovikova, L. V. Savitskaya, S. A. Badarin, S. V. Generalov, N. I. Kostyleva, N. A. Sharapova, Yu. V. Ivanov
Được phát hành 2012-09-01We presented the data concerning optimization of presentation and consumer container of anti-rabies immunoglobulin obtained, from horse serum.. During the experiments, ampoules and. flasks were used for primary packaging. They were filled within 5 ml of the preparation of freeze-drying. Comparative...
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12
ISOLATION OF GLICOPROTEID FROM THE FIXED RABIES VIRUS, STRAIN «MOSCOW 3253», AND CONSTRUCTING OF DOT-IMMUNOASSAY DIAGNOSTICUM ON ITS BASIS Bằng N. A. Sharapova, M. N. Kireyev, E. G. Abramova, S. V. Generalov, Zh. V. Matveyeva, I. M. Zhulidov, N. E. Tereshkina, E. A. Mikheyeva, L. V. Savitskaya, A. V. Gayeva, A. K. Nikiforov
Được phát hành 2012-09-01Described here are the results of glicoproteid isolation from the fixed rabies virus, strain «Moscow 3253», using non-ionic detergent with subsequent chromatographic purification. The obtained antigen was demonstrated to be applicable as immunoreagent for construction of diagnosticum, by means of co...
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13
Characteristics of <i>Vibrio cholerae</i> O1 CTX<sup>+</sup> Strain Isolated from the Temernik River in Rostov-on-Don in 2023 Bằng E. V. Monakhova, V. D. Kruglikov, A. S. Vodop’yanov, Ya. M. Krasnov, S. D. Katyshev, N. A. Sharapova, L. P. Alekseeva, E. A. Men’shikova, V. V. Evdokimova, M. I. Ezhova, A. K. Noskov
Được phát hành 2024-07-01The aim of the work was to determine the pheno- and genotypic features of the aquatic CTX+ strain of Vibrio cholerae isolated in 2023 and a comparative bioinformatic analysis of whole-genome sequencing data.Materials and methods. Whole-genome sequencing was performed on MiSeq (Illumina) and MinIon O...
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14
Molecular-Genetic and Phenotypic Peculiarities of Plague Agent Strains Isolated in Vietnam Bằng L. M. Kukleva, K. A. Nikiforov, Zh. V. Al’khova, N. I. Romanov, N. Yu. Nosov, A. V. Fadeeva, A. N. Malakhaeva, N. A. Sharapova, Z. L. Devdariani, Yu. A. Popov, G. A. Eroshenko
Được phát hành 2017-12-01Objective of the study is to investigate phenotypic and molecular-genetic features and perform whole genome sequencing of Y. pestis strains isolated in Vietnam. Materials and methods. Studied were phenotypic and genotypic peculiarities of 20 plague agent strains isolated in different prefectures of...
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15
Production of Lyophilized Preparation of Anti-Rabies Immunoglobulin and Examination of Its Main Properties Bằng E. G. Abramova, N. N. Kochkalova, A. K. Nikiforov, A. Yu. Butyrskii, Yu. V. Ivanov, N. V. Sinitsyna, T. A. Mikheeva, L. N. Minaeva, M. V. Galkina, L. V. Savitskaya, S. V. Generalov, M. N. Kireev, N. A. Sharapova
Được phát hành 2011-04-01Presented are data on examination of physical, chemical and biological characteristics of lyophilized preparation of geterologous anti-rabies immunoglobulin. Dry and original liquid forms of immunoglobulin were comparatively analyzed. The lyophilized form was shown to preserve main properties during...
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Specific Appurtenance, Numbers, and Dynamics of Interaction of Acanthamoeba from Soils of Gorno-Altai High-Mountain Plague Focus with Yersinia pestis Strains Bằng E. G. Oglodin, O. A. Morozov, K. A. Nikiforov, L. M. Kukleva, N. A. Sharapova, E. N. Rozhdestvensky, G. Kh. Bazarova, A. V. Denisov, P. P. Sanarov, E. G. Tokmakova, M. A. Makashova, V. G. Germanchuk, G. A. Eroshenko, V. V. Kutyrev
Được phát hành 2017-12-01Objective of the study is to analyze the appurtenance, numbers, and dynamics of interaction of acanthamoeba from soils of GornoAltai plague focus with Yersinia pestis 367 strain, isolated in 2016 in enzootic territory of this focus. Materials and methods. Utilized were soil amoeba from Gorno-Altai h...
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17
Non-Waste Alternative Technologies in the Production of Heterologous Anti-Rabies Immunoglobulin Bằng I. M. Zhulidov, E. G. Abramova, A. K. Nikiforov, M. V. Antonycheva, I. V. Shul'gina, O. A. Lobovikova, N. I. Vakhrushina, A. D. Belousov, S. A. Eremin, M. N. Kireev, N. A. Sharapova, L. V. Savitskaya, T. A. Mikheeva, L. N. Minaeva, M. V. Galkina, R. A. Svintsov, T. V. Alenkina, Yu. G. Vasin, G. V. Bazlov
Được phát hành 2011-08-01Presented is a comprehensive approach to utilization of the wastes that appear in the process of heterologous anti-rabies immunoglobulin production (packed red cells, fibrin, and alcohol-containing products). Specific immunoglobulin is extracted from the surface of red blood cells using desorption t...
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