Search Results - LI Jian-rong
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Study on bivalent attenuated vaccine of infectious bursal disease virus variants
Published 2000-05-01Get full textFor detecting their immunogenicity and security, infectious bursal disease virus (IBDV) “JN” and “JH” bivalent live vaccine were vaccinated into 14 day old SPF chickens at three different doses (6 000 TCID<sub>50</sub>、10 000 TCID<sub>50</sub>、20 000 TCID<sub>50</sub...
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Expression of the C-terminal 26 kDa fragment of Helicobacter pylori UreB in Escherichia coli and its immunity
Published 2006-07-01Get full textA 720 bp fragment of ureB encoding the urease beta subunits of Helicobacter pylori was cloned into an expression vector (pAMJ399) and expressed in Escherichia coli JM109 cells. The recombinant rUreB-E fractions of UreB displayed molecular masses of approximate 26 kDa estimated by SDS-PAGE. Western b...
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Pathogenicity and immunogenicity of recombinant methyltransferase-defective vesicular stomatitis virus
Published 2011-07-01Get full textTo prevent the vesicular stomatitis virus (VSV) disease, using a panel of recombinant VSV (E1764A, S1827A, Y1650A, and F1691A) that were defective in mRNA cap methylation as vaccine to inoculate five-week-old BALB/c female mice through oral route with 1×10<sup>6</sup> PFU, the pathogenic...
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Enhancement of immunogenicity of DNA vaccine against infectious bursal disease virus by co-delivery with oral vaccine encoding chicken interleukin-2
Published 2007-05-01Get full textMice and 14-day-old chickens were orally inoculated with the recombinant attenuated Salmonella typhimurium strain ZJ111 carrying pCI-IL-2 expression plasmid encoding the Interleukin II protein. The results showed the ZJ111/pCI-IL-2 was relatively safe. Fourteen-day-old non-immunized chickens were co...
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Expression of chicken interleukin-2(IL-2) in E. coli. and preparation of its polyclonal antibody
Published 2003-01-01Get full textInterleukin-2 (IL-2) is known to play a number of functions in the modulation of immunity. In this study the chicken IL-2 gene was cloned into a prokaryon vector pET-28b and the recipient cell of E. coli. BL21 was transformed with the recombinant expression vector pET-28b-IL-2. IL-2 was expressed un...
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Construction of eukaryotic expression vector of Interleukin-2 gene from Xiaoshan Chicken and its expression in Vero cells
Published 2003-11-01Get full textIn previous study IL-2 gene of Xiaoshan Chicken has been cloned by our laboratory, and in this study the cDNA of IL-2 was amplified and inserted into the eukaryotic expression vector pCI. The resulted recombinant plasmid pCI-IL-2 was transformed by electroporation into an attenuated Salmonella typhi...
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Study on the Immunogenicity of Infectious Bursal Disease Virus VP2 proteins Expressed in Silkworm
Published 2000-01-01Get full textThe VP2 protein of infectious bursal disease virus (IBDV) was expressed in spring and autumn silkworm larvae as detected by Western blot and ELISA. Non-immunized 18-day-old chickens were vaccinated via subcutaneous injection with a vaccine preparation made from the lymph of spring silkworms containi...
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