Search Results - JIN Yong-feng
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Recent advances in baculoviral inhibitor of apoptosis
Published 2001-05-01Get full textApoptosis is an active process of cellular self-destruction that is involved in normal development. Two kinds of anti-apoptosis genes p35 and iap have been identified in baculovirus genome. Both of them function at different point in the death pathway. Extensive studies have been made about the stru...
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Extraction of mitochondrial DNA from cytoplasmic male sterile and maintainer lines of tuber mustard (Brassica juncea var tumida Tsen et Lee)
Published 2000-05-01Get full textMitochondrial (mt) DNA has been extracted from paired lines of cytoplasmic male sterile and maintainer lines of tuber mustard based on sucrose shelf. Evaluation of mtDNA by agrose gel electrophoresis showed the good quality DNA which can meet the need of the following research work: the mtDNA sample...
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Expression of 6 × his-hGM-CSF fusion gene in larvae of silkworm (Bombyx mori) using recombinant baculovirus
Published 2005-09-01Get full textSix × his-hGM-CSF fusion gene was inserted in silkworm baculovirus transfer vector pBacPAK8 to construct the recombinant transfer vector pBacPAKHis-GM-CSF. The recombinant transfer vector DNA and linear virus Bm-BacPAK6 DNA were co-transfected into BmN cells. The homologous recombination occurred in...
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Research on the production of useful protein using silkworm (Bombyx mori) as a bioreactor
Published 2001-03-01Get full textSummarized the basic principle, production steps, achievements of expression foreign genes and pharmaceuticals development using Silkworm/BmNPV Expression System, and introduced technological development of the mass production of useful protein in silkworm, and revealed the bright prospect.
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