Search Results - JIANG Dong-hua
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Effects of five fungicides on mycelial growth of L. edodes, P. ostreates, F. velutipes and contaminated mold
Published 2001-05-01Get full textEffects of five fungicides on the hypha growth of L. edodes, P. ostreates, F. velutipes and contaminated mold were studied. The results showed that the inhibition with different kinds and treatment concentrations of fungicides on the hypha growth of three edible fungi and contaminated mold had signi...
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Cloning of cryptogein gene and construction of its plant expression vector
Published 2002-11-01Get full textThe cryptogein gene was obtained by PCR amplification from genomic DNA of Phytophthora cryptogea and verified by DNA sequencing. A promoter of a rice phenylalanine ammonia-lyase (PAL) gene was selected to control the expression of cryptogein gene, because the PAL promoter is low-level constitutive,...
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Transformation of CryK13V gene into protoplasts of Trichoderma viride
Published 2006-05-01Get full textThe lysine in site 13 of cryptogein protein was mutated to valine (K13V) through PCR site-directed mutagenesis. The mutant fragment (CryK13V) was confirmed by enzyme digestion and DNA sequencing. The CryK13V gene was expressed in Trichoderma viride, with constructed vector pCSNTCCm. Transformation w...
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