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    Highly efficient genome editing in Bacillus subtilis via miniature DNA nucleases IscB

    Highly efficient genome editing in Bacillus subtilis via miniature DNA nucleases IscB by Jie Gao, Hengyi Wang, Jingtao Sun, Hongjie Tang, Yuhan Yang, Qi Li

    Published 2025-12-01

    Existing CRISPR-based genome editing techniques for Bacillus subtilis (B. subtilis) are limited due to the large size of the cas gene. IscB, a recently reported DNA nuclease, is one-third the size of Cas9, making it a potential tool for genome editing; however, its application in B. subtilis remains...

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