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    Optimizing expression of LZ-8 protein and primary assaying of its immunomodulatory activity

    Optimizing expression of LZ-8 protein and primary assaying of its immunomodulatory activity by HUANG Jun, ZHANG Ling-xia, HU Song-nian, YU Jun

    Published 2008-01-01

    To obtain sufficient recombinant LZ-8 protein (rLZ-8) for assaying its immunosuppressive activity, 12 overlapping oligonucleotides optimized by the usage of the favorite codons in E. coli were synthesized and connected to form a new LZ-8 gene by PCR. The recombinant plasmid pET-28b_lz8 containing th...

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