Search Results - FANG Wei-huan
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PCR detection of haemolysin gene of Streptococcus suis type 2
Published 2004-01-01Get full textA PCR assay for rapid and sensitive detection of haemolysin of Streptococcus suis type 2 was established. The PCR primers based on the haemolysin of S. suis type 2 succeeded in amplifying a 1502 bp PCR product. The PCR product could be digested into two fragments of 869 bp and 633 bp by EcoR I. With...
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Crude extraction and purification of IgY against Aeromonas hydrophila and its antibacterial activity in vitro
Published 2005-07-01Get full textUsing water-dilution to extract crude IgY from egg yolk of hens immunized with an inactivated Aeromonas hydrophila and then introducing saturated ammonium sulfate solution to purify the IgY from crude extract, this study evaluated the immunocompetence recovery and antibacterial activity of IgY in vi...
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Prokaryotic expression of structural domains of the Newcastle disease virus fusion protein and analyses of their antigenic epitopes
Published 2006-01-01Get full textThe gene fragments Fa, Fb and Fa-l-Fb coding for the antigenic structural domains of the Newcastle disease virus (NDV) fusin protein were amplified from the recombinant plasmid containing full length NDV F gene by overlap extension PCR with specific primers. The genes were inserted between SalⅠ and...
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Construction of eukaryotic expression vector of Interleukin-2 gene from Xiaoshan Chicken and its expression in Vero cells
Published 2003-11-01Get full textIn previous study IL-2 gene of Xiaoshan Chicken has been cloned by our laboratory, and in this study the cDNA of IL-2 was amplified and inserted into the eukaryotic expression vector pCI. The resulted recombinant plasmid pCI-IL-2 was transformed by electroporation into an attenuated Salmonella typhi...
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Attenuated Salmonella typhimurium as a carrier for prokaryotic and eukaryotic expression vectors
Published 2006-05-01Get full textAn attenuated Salmonella enterica serovar typhimurium strain as the host for recombinant prokaryotic and eukaryotic vectors containing the enhanced green fluorescent protein (eGFP) gene was examined by fluorimetric and fluorescent microscopic methods. Expression of eGFP was dependent on the concentr...
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Optimum conditions of in vitro proliferation of splenic lymphocyte from Chinese soft-shelled turtle (Trionyx sinensis)
Published 2008-07-01Get full textIn order to optimize the conditions for in vitro proliferation of splenic lymphocyte of Chinese Soft-shelled turtle (Trionyx sinensis), four main parameters were tested including different kinds and concentrations of mitogens, cell density and incubation time. By colorimetric method utilizing MTT [3...
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Serological and virulence factors analysis of Escherichia coli isolates causing post-weaning diarrhea in intensive pig farms
Published 2006-09-01Get full textIn order to determine the prevalent serotypes and virulence factors of pathogenic Escherichia coli isolates from piglets with post-weaning diarrhea (PWD) in intensive pig farms of Zhejiang Province, rectum swabs from piglets with PWD in intensive pig farms were collected for isolations of pathogenic...
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Construction of the recombinant Listeria monocytogenes mutant expressing green fluorescent protein
Published 2005-09-01Get full textA recombinant Listeria monocyogenes mutant expressing green fluorescent protein (GFP) gene was constructed by homologous recombination. The target gene gfp was fused to the actA gene fragment downstream of its promoter and signal sequence. PCR amplification and restriction digestion of the PCR produ...
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Surveillance of resistant pathogenic E. coli from post-weaning diarrhea on intensive pig farms in Zhejiang province
Published 2004-09-01Get full textAntimicrobial susceptibility testing was performed on 60 clinical Escherichia coli isolates from pigs with post-weaning diarrhea in 10 intensive pig farms in Zhejiang province. The results showed that all the 60 pathogenic E. coli isolates have developed various degrees of resistances to 21 commonly...
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