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    High-level expression and purification of Plutella xylostella acetylcholinesterase in Pichia pastoris and its potential application

    High-level expression and purification of Plutella xylostella acetylcholinesterase in Pichia pastoris and its potential application by Niu LIU, Jun CHE, Duo LAI, Jin-jun WEN, Han-hong XU

    Published 2017-06-01

    The acetylcholinesterase 2 (AChE2) cloned from Plutella xylostella was first successfully expressed in methylotrophic yeast Pichia pastoris GS115. One transformant with high-level expression of the recombinant AChE (rAChE, 23.2 U mL−1 in supernatant) was selected by plating on increasing concentrati...

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