Результаты поиска - Chang Long
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Coupling of green building construction based on particle Swarm optimizing neural network algorithm
Опубликовано 2025-01-01Полный текстIn the continuous development of the green building industry, construction safety management faces increasing challenges, particularly in safety and environmental protection, which requires precise evaluation and control. Therefore, this study proposes a coupling analysis method for green building c...
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Jasmonic acid and ethylene signaling pathways participate in the defense response of Chinese cabbage to Pectobacterium carotovorum infection
Опубликовано 2021-05-01Полный текстChinese cabbage (Brassica rapa subsp. pekinensis) suffers from soft rot disease caused by Pectobacterium carotovorum (Pc). To uncover the mechanisms underlying the defense response of Chinese cabbage to Pc, we constructed a suppression subtractive hybridization (SSH) library from Pc-infected cabbage...
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The Minimal Active Fragment of the Cry1Ai Toxin is Located Between 36I and 605I
Опубликовано 2014-05-01Полный текстThe novel cry1Ai gene that cloned from Bacillus thuringiensis strain SC6H8 encoded a protein exhibiting strong toxicity against Plutella xylostella and Chilo suppressalis in our previous study. Using the available information for the active fragments of other Cry toxins, eight truncated fragments we...
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Identification of similar transcriptional regulatory mechanisms in multiple cry genes in Bacillus thuringiensis HD12
Опубликовано 2017-01-01Полный текстBacillus thuringiensis subspecies morrisoni strain HD12, whose genome harbors multiple insecticidal protein-encoding genes, includes eight cry genes, as indicated by genome sequencing. This strain produces crystals that are toxic to lepidopteran species. These crystal inclusions were purified by suc...
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Complete genome sequence of Bacillus thuringiensis Bt185, a potential soil insect biocontrol agent
Опубликовано 2017-03-01Полный текстBacillus thuringiensis Bt185 and its insecticidal spectrum-expanded engineering strains are considered as potential biocontrol agents to soil insect Holotrichia parallela, Holotrichia oblita or Anomala corpulenta. Here we reported the complete genome of strain Bt185, it harbors eight plasmids, and p...
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Comparison and optimization of the method for Cry1Ac protoxin preparation in HD73 strain
Опубликовано 2015-08-01Полный текстBacillus thuringiensis is one of the most widely used bioinsecticides, and cry gene is the major insecticidal gene. Because Cry1Ac protein shows strong toxicity against many lepidopteran species, it has been applied widely in spraying products and transgenic Bt-crops. The preparation of Cry protoxin...
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The Influence of Bt-Transgenic Maize Pollen on the Bacterial Diversity in the Midgut of Chinese Honeybees, Apis cerana cerana
Опубликовано 2013-03-01Полный текстUsing culture-independent technique polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and conventional culture techniques, ecological risk of transgenic maize pollen on gut bacteria of the Chinese honeybee, Apis cerana cerana, was assessed. Honeybees were fed with Bt-trans...
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Host status of Brachypodium distachyon to the cereal cyst nematode
Опубликовано 2018-02-01Полный текстCereal cyst nematode (Heterodera avenae, CCN) distributes worldwide and has caused severe damage to cereal crops, and a model host will greatly aid in the study of this nematode. In this research, we assessed the sensitivity of 25 inbred lines of Brachypodium distachyon to H. avenae from Beijing, Ch...
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Application of droplet digital PCR in detection of seed-transmitted pathogen Acidovorax citrulli
Опубликовано 2020-02-01Полный текстBacterial fruit blotch caused by Acidovorax citrulli is a serious threat to cucurbit industry worldwide. The pathogen is seed-transmitted, so seed detection to prevent distribution of contaminated seed is crucial in disease management. In this study, we adapted a quantitative real-time PCR (qPCR) as...
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