Ngā hua rapu - Chang Long

  • E whakaatu ana i te 1 - 9 hua o te 9
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  1. 1

    Coupling of green building construction based on particle Swarm optimizing neural network algorithm Wang Leigang, Li Shaohua, Wang Liang, Zhang Zheng, Zhou Yuchen, Chang Long

    I whakaputaina 2025-01-01

    In the continuous development of the green building industry, construction safety management faces increasing challenges, particularly in safety and environmental protection, which requires precise evaluation and control. Therefore, this study proposes a coupling analysis method for green building c...

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  2. 2

    Jasmonic acid and ethylene signaling pathways participate in the defense response of Chinese cabbage to Pectobacterium carotovorum infection Chang-long CHEN, Fang YUAN, Xiao-ying LI, Rong-cai MA, Hua XIE

    I whakaputaina 2021-05-01

    Chinese cabbage (Brassica rapa subsp. pekinensis) suffers from soft rot disease caused by Pectobacterium carotovorum (Pc). To uncover the mechanisms underlying the defense response of Chinese cabbage to Pc, we constructed a suppression subtractive hybridization (SSH) library from Pc-infected cabbage...

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  3. 3

    The Minimal Active Fragment of the Cry1Ai Toxin is Located Between 36I and 605I Zi-shan ZHOU, Hui-yan LIN, Ying LI, Chang-long SHU, Fu-ping SONG, Jie ZHANG

    I whakaputaina 2014-05-01

    The novel cry1Ai gene that cloned from Bacillus thuringiensis strain SC6H8 encoded a protein exhibiting strong toxicity against Plutella xylostella and Chilo suppressalis in our previous study. Using the available information for the active fragments of other Cry toxins, eight truncated fragments we...

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  4. 4

    Identification of similar transcriptional regulatory mechanisms in multiple cry genes in Bacillus thuringiensis HD12 Zhi-ru SONG, Qi PENG, Chang-long SHU, Jie ZHANG, Dong-mei SUN, Fu-ping SONG

    I whakaputaina 2017-01-01

    Bacillus thuringiensis subspecies morrisoni strain HD12, whose genome harbors multiple insecticidal protein-encoding genes, includes eight cry genes, as indicated by genome sequencing. This strain produces crystals that are toxic to lepidopteran species. These crystal inclusions were purified by suc...

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  5. 5

    Complete genome sequence of Bacillus thuringiensis Bt185, a potential soil insect biocontrol agent Yan-qiu LI, Chang-long SHU, Yue-ming SHAN, Li-li GENG, Fu-ping SONG, Jie ZHANG

    I whakaputaina 2017-03-01

    Bacillus thuringiensis Bt185 and its insecticidal spectrum-expanded engineering strains are considered as potential biocontrol agents to soil insect Holotrichia parallela, Holotrichia oblita or Anomala corpulenta. Here we reported the complete genome of strain Bt185, it harbors eight plasmids, and p...

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  6. 6

    Comparison and optimization of the method for Cry1Ac protoxin preparation in HD73 strain Zi-shan ZHOU, Su-juan YANG, Chang-long SHU, Fu-ping SONG, Xue-ping ZHOU, Jie ZHANG

    I whakaputaina 2015-08-01

    Bacillus thuringiensis is one of the most widely used bioinsecticides, and cry gene is the major insecticidal gene. Because Cry1Ac protein shows strong toxicity against many lepidopteran species, it has been applied widely in spraying products and transgenic Bt-crops. The preparation of Cry protoxin...

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  7. 7

    The Influence of Bt-Transgenic Maize Pollen on the Bacterial Diversity in the Midgut of Chinese Honeybees, Apis cerana cerana Wei-yu JIANG, Li-li GENG, Ping-li DAI, Zhi-hong LANG, Chang-long SHU, Yi LIN, Ting ZHOU, Fu-ping SONG, Jie ZHANG

    I whakaputaina 2013-03-01

    Using culture-independent technique polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and conventional culture techniques, ecological risk of transgenic maize pollen on gut bacteria of the Chinese honeybee, Apis cerana cerana, was assessed. Honeybees were fed with Bt-trans...

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  8. 8

    Host status of Brachypodium distachyon to the cereal cyst nematode Chang-long CHEN, Shu-sen LIU, Qian LIU, Jun-hai NIU, Pei LIU, Jian-long ZHAO, Zhi-yong LIU, Hong-jie LI, Heng JIAN

    I whakaputaina 2018-02-01

    Cereal cyst nematode (Heterodera avenae, CCN) distributes worldwide and has caused severe damage to cereal crops, and a model host will greatly aid in the study of this nematode. In this research, we assessed the sensitivity of 25 inbred lines of Brachypodium distachyon to H. avenae from Beijing, Ch...

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  9. 9

    Application of droplet digital PCR in detection of seed-transmitted pathogen Acidovorax citrulli Yu LU, Hai-jun ZHANG, Zi-jing ZHAO, Chang-long WEN, Ping WU, Shun-hua SONG, Shuan-cang YU, Lai-xin LUO, Xiu-lan XU

    I whakaputaina 2020-02-01

    Bacterial fruit blotch caused by Acidovorax citrulli is a serious threat to cucurbit industry worldwide. The pathogen is seed-transmitted, so seed detection to prevent distribution of contaminated seed is crucial in disease management. In this study, we adapted a quantitative real-time PCR (qPCR) as...

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