Søgeresultater - CHENG Li-qin
-
1
Variation of nucleocapsid protein gene of infectious bronchitis virus and its expression in Escherichia coli
Udgivet 2003-01-01Få fuldtekstNucleocapsis (N) protein gene is one of the major structural protein genes of infectious bronchitis virus (IBV). Our previous studies have indicated that IBV strain ZJ971 causes proventriculus lesions while strains N and X result in chicken nephritis. This research was undertaken to investigate thei...
Article -
2
Cloning and sequencing of the S gene of H52 strain from avian infectious bronchitis virus
Udgivet 2002-05-01Få fuldtekstVirion RNA from H52 strain of avian Infectious Bronchitis were extracted from the allantoic fluid of inoculated embryonated eggs and used as template for cDNA synthesis by reverse transcription with 3'-primer. Polymerase chain reaction was performed with two primers which span the S gene, and a...
Article -
3
The construction of plant expression vector of S1 gene of avian infectious branchitis virus
Udgivet 2001-05-01Få fuldtekstThe S1 gene of avian infectious bronchitis virus (ZJ971 strain) was amplified by polymerase chain reaction with special primers containing XbalI and BamHI. The purified PCR product was digested by the restricted enzyme XbalI and BamHI. The digested and purified S1 gene of infectious bronchitis virus...
Article