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    Cloning and expression of α-acetolactate decarboxylase gene from Enterobacter aerogenes in Escherichia coli

    Cloning and expression of α-acetolactate decarboxylase gene from Enterobacter aerogenes in Escherichia coli by ZHENG Xiao-dong, CHEN Xin-ai, QI Yi-jun

    Published 2000-05-01

    The ALDC gene was cloned by PCR using Enterobactr aerogenes chromosome DNA as template and got 0.78 kb DNA fragment. The fragment was inserted directionally into a prokaryotic expression vector pQE-30 with high efficiency. The ALDC gene was overexpressed in E. coli M15, and the production of ALDC wa...

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