Risultati della ricerca - CHEN Li-geng
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1
Studies of Chinese bayberry fruits on some physiological characters during the storage
Pubblicazione 2001-03-01TestoFruit of Chinese bayberry (Myrica rubra (Lour.) Sieb et Zucc) ‘Biqi’ culture were used in this study. The fruits were stored at the temperature of (21±1)℃, (11±1)℃ or (1±1)℃. By investigating the respiratory rate, ethylene production, POD, PG, soluble pectin and cellulose during the storage, the con...
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2
Studies on cold tolerance in navel orange strains
Pubblicazione 2000-09-01TestoThe cold tolerance of 6 navel orange strains (Citrus Sinensis (L) Osbeck.) was determined. The result showed that through 10 hours treatment at -7℃ the relative conductivity of 954 navel orange was less than 50%, and LT<sub>50</sub> of it was -6.58 ℃. 954 navel orange was also found to h...
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3
Cloning and sequence analysis of P<sub>38</sub> gene of Grapevine fanleaf virus Hangzhou isolated
Pubblicazione 2003-01-01TestoTo confirm the characteristics and function of P<sub>38</sub> in the viral particles movement, the cDNA fragment of P<sub>38</sub> gene of Hangzhou isolate of Grapevine fanleaf virus (GFLV-H) was amplified by RT-PCR with specific primers to the RNA<sub>2</sub> seq...
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4
Cloning of coat protein gene of Grapevine fanleaf virus Hangzhou isolate and construction of its plant expression vector
Pubblicazione 2002-11-01TestoThe coat protein (CP) gene cDNA of Grapevine fanleaf virus (GFLV) Hangzhou isolate was amplified from total viral RNA by RT-PCR, then inserted into pGEM-T-easy Vector. Sequence analysis showed that this gene contained 1515 nucleotide acids encoding 504 amino acids. The nucleotide sequence and deduce...
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5
Development and studies on LEAFY genetic transformation system with high efficiency in commercial grape (Vitis vinifera L. var muscat)
Pubblicazione 2001-09-01TestoTransgenic plants appear to be a new way to grape improvement and a powerful tool for studying gene functions. Using a direct high frequency shoot regeneration system suitable for genetic transformation was established successfully in this study, which was the base for the following research of cons...
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Study on Genetic diversity of Frankia strains from symbiotic nodules of Myrica rubra
Pubblicazione 2002-11-01TestoTen isolates of Frankia from symbiotic nodules of Myrica rubra were studied on their phenotypes and RFLP analyses of their PCR products amplified from 16S rDNA by restriction enzymes Hinf I and Hae Ⅲ. The results showed that there are differences not only in their phenotypes but also in their gene t...
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7
Cloning and analysis of LFY-like gene from Citrus
Pubblicazione 2001-05-01TestoThe homologous DNA fragment of LFY was cloned from citrus genomic DNA that had been isolated from Sweet orange (Citrus sinensis (L) Osbeck) using Uneven PCR. Analysis of the nucleotide sequence indicated that homology was up to 90% between it and NFY, another LFY-like gene, which implied that they w...
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8
Isolation and cultural characteristics of Frankia symbiosed with Myrica rubra
Pubblicazione 2003-11-01TestoThree endophytes isolated from root nodules of Myrica rubra var. biji were identified as Frankia sp. and designated as Mpc11, Mpc12 and Mpa11 based on results of microscopic examination, inoculation, nitrogenase activitity measurement and Gram-staining. Characteristics of behavior of the three isola...
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