Search Results - A. V. Shchulkin

Relevance. Arterial hypertension (AH) is widespread throughout the world. Unfortunately, only a small proportion of patients achieve target blood pressure (BP). One of the possible reasons for the ineffectiveness of AH treatment is the pharmacokinetics of antihypertensive drugs.Objective. To test va...

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the modern ideas of the role of free radical processes in the pathogenesis of age-related macular degeneration (AMD) are consid- ered. Data of large randomized clinical trials on application of antioxidants for prevention and therapy AMD are provided. Possibility of the differential application of a...

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Relevance. BCRP is an efflux transporter protein that plays an important role in the pharmacokinetics of a wide range of drugs. The BCRP activity in vitro experiments is assessed by the transport of transporter protein substrates (methotrexate, etc.) across the bilipid membrane of cells overexpressi...

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Restenosis of the reconstruction zone is one of the main postoperative complications of vascular reconstructions, which develops in 18–40% of cases and leads to an increase in the risk of limb loss to 20–25%. The search for new markers for predicting the development of this complication is an urgent...

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Relevance. Ethylmethylhydroxypyridine succinate (EMHPS) is a reference domestic drug with pronounced antioxidant and antihypoxic activity. P-Glycoprotein (Pgp) is an ATP-dependent transport protein localized in tissue barriers and protecting cells and organs from the effects of xenobiotics. Being ex...

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The aim of the article is to evaluate the ABCB1 protein inhibition in the blood-brain barrier to increase the effectiveness of neuroprotective therapy for focal and global cerebral ischemia.Materials and methods. The work was performed on 103 male Wistar rats. In the 1st group (n=33), the neuroprote...

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Promising metabolites of potato tuberosum (Solanum tuberosum L., f. Solanaceae) are α-solanine, α-chaconine and their aglycone solanidine.The aim of the work was to develop and validate methods for a quantitative analysis of α-solanine, α-chaconine and solanidine in dry extracts from the potato tube...

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The investigation of pharmacokinetics of marker substrates of carrier protein P-glycoprotein (Pgp, ABCB1-protein) including fexofenadine, is one of the methods of its functional activity evaluation.The aim of the study was to work out the HPLC methods of the quantitative determination of fexofenadin...

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This review addresses the function of the protein-transporter glycoprotein-P (Pgp, ABCB1 protein) and the expression of its encoding gene MDR1/mdr1 throughout the gestational period in humans and animals. We discuss the relationship between the transporter function and the hormone fluctuations durin...

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Mitoxantrone is a marker substrate of breast cancer resistance protein (BCRP). BCRP is involved in a number of pharmacokinetic drug-drug interactions. The transporter’s possible saturability makes it advisable to use low concentrations of mitoxantrone for in vitro studies. Consequently, mitoxantrone...

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Research justification. Corneal ulcer is a common corneal pathology dangerous because of its complications, which occur in 2.5–37.9 % of cases despite modern treatment. Thus, the development of novel methods improving the results of corneal ulcer treatment is very important.Research objective was to...

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One way to analyze the activity of the ABCB1 protein is to assess the accumulation of its substrate fexofenadine (F.) inside the test cells. The goal is to develop and validate a method for the quantitative analysis of F. in Caco-2 cell lysate using HPLC-MS/MS. Materials and methods. Caco-2 cell lys...

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Introduction. Breast cancer resistance protein (BCRP) is an efflux membrane transporter that controls the pharmacokinetics of a large number of drugs. Its activity may change when taking some endo- and exogenous substances, thus making it a link in drug interactions.Aim. The aim of the study was to...

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